N(alpha)-fmoc-N(epsilon)-(2-chloro-Z)-L-lysine(CAS# 133970-31-7)

Fmoc-(2-chlorobenzyloxycarbonyl) lysine ke motsoako oa amino acid o sebelisoang hangata o nang le litšobotsi tse latelang:1. Ponahalo: phofo e tšoeu ea kristale;
2. foromo ea molek'hule: C26H24ClNO5;
3. Boima ba molek'hule: 459.92g / mol;
4. Solubility: E qhibiliha ka har'a li-solvents tse kang dimethyl sulfoxide (DMSO), dimethyl formamide (DMF), dichloromethane, joalo-joalo, e sa qhibiliheng metsing;
5. Sebaka sa ho qhibiliha: hoo e ka bang 170-175 ° C. Tšebeliso e ka sehloohong ea Fmoc-(2-chlorobenzyloxycarbonyl) lysine ke sehlopha se sireletsang le se sebetsang ka har'a motsoako oa polypeptides. Sehlopha sa eona sa carboxyl se ka kengoa tšebetsong ho theha ester, ebe e kenella karabelo ea condensation ka masala a amino acid ho etsa ketane ea polypeptide. Sehlopha sa Fmoc se ka tlosoa habonolo ka mor'a hore karabelo e phethoe ho pepesa karolo e sirelelitsoeng ea amino.

Mokhoa oa ho lokisa Fmoc-(2-chlorobenzyloxycarbonyl) lysine ka kakaretso o kenyelletsa mehato e latelang:

1. ho sebetsana le lysine le N-hydroxybutyrimide (Pbf) ho hlahisa sehlopha se sireletsang;
2. ho arabela motsoako oa lysine-Pbf ka joala ba 2-chlorobenzyl ho etsa Fmoc-(2-chlorobenzyloxycarbonyl) lysine;
3. Sehlahisoa se ntšoa ka solvent e loketseng 'me se hloekisoa ka crystallization ho fumana sehlahisoa se hloekileng.

Mabapi le tlhahisoleseding ya polokeho, Fmoc-(2-chlorobenzyloxycarbonyl) lysine ke sehlahiswa sa dikhemikhale mme ho lokela ho nkuwa mehato e nepahetseng ya polokeho. Lisebelisoa tse nepahetseng tsa ts'ireletso, joalo ka liatlana tsa lab, likhalase le liaparo tsa lab, li lokela ho apesoa nakong ea liteko. Qoba ho hema phofo kapa tharollo, qoba ho kopana le letlalo le mahlo. Haeba ho ka etsahala hore u kopane ka phoso, hlatsoa hang-hang ka metsi a mangata 'me u batle thuso ea bongaka. Netefatsa hore e sebelisoa sebakeng se sireletsehileng sa laboratori le ho bolokoa ka nepo ho thibela likotsi.