Isopropyl-beta-D-thiogalactopyranoside (CAS#367-93-1)
Kotsi le Polokeho
| Likhoutu tsa Kotsi | R19 - E ka etsa peroxides e phatlohang R40 - Bopaki bo fokolang ba phello ea kankere R66 – Ho pepeseha kgafetsa ho ka baka ho omella ha letlalo kapa ho peperana R36/37/38 – Ho tena mahlo, tsamaiso ya ho hema le letlalo. |
| Tlhaloso ea Tšireletseho | S23 - Se ke oa hema mouoane. S24/25 - Qoba ho kopana le letlalo le mahlo. S36/37 - Apara liaparo tse loketseng tsa tšireletso le liatlana. S22 - U se ke ua hema lerole. S36/37/39 - Apara liaparo tse loketseng tsa tšireletso, liatlana le tšireletso ea mahlo / sefahleho. S27 - Tlosa hang-hang liaparo tsohle tse silafetseng. S26 - Ha u kopana le mahlo, hlatsoa hang-hang ka metsi a mangata 'me u batle keletso ea bongaka. |
| WGK Jeremane | 3 |
| LIKHOUTU FLUKA BRAND F | 10 |
| TSCA | Ee |
| Khoutu ea HS | 29389090 |
Selelekela
IPTG ke ntho e susumetsang ts'ebetso ea β-galactosidase. Ho ipapisitsoe le tšobotsi ena, ha DNA ea vector ea letoto la pUC (kapa DNA e 'ngoe ea vector e nang le lacZ gene) e fetoloa ka lisele tsa lacZ tsa ho hlakola e le moamoheli, kapa ha DNA ea vector ea M13 phage e fetisoa, haeba X-gal le IPTG li eketsoa. ho ea bohareng ba poleiti, ka lebaka la α-complementarity ea β-galactosidase, gene recombinant e ka khethoa habonolo ho latela hore na likolone tse tšoeu (kapa li-plaque) lia hlaha. Ntle le moo, e ka sebelisoa hape e le sesupo sa polelo bakeng sa li-vector tsa polelo tse nang le baetsi ba joalo ka lac kapa tac. E qhibiliha ka metsing, methanol, ethanol, e qhibilihang ka har'a acetone, chloroform, ha e qhibilihe ka har'a ether. Ke inducer ea β-galactosidase le β-galactosidase. Ha e na hydrolyzed ka β-galactoside. Ke tharollo ea substrate ea thiogalactosyltransferase. E entsoe: IPTG e qhibiliha ka metsing, ebe e sterilized ho lokisa tharollo ea polokelo (0 · 1M). Khatiso ea ho qetela ea IPTG ka poleiti ea pontšo e lokela ho ba 0 · 2mM.
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